Comparative study of the removal of sulfate by UASB in light and dark environment.

At present, the application of sewage treatment technologies is restricted by high sulfate concentrations. In the present work, the sulfate removal was biologically treated using an upflow anaerobic sludge blanket (UASB) in the absence/presence of light. First, the start-up of UASB for the sulfate removal was studied in terms of COD degradation, sulfate removal, and effluent pH. Second, the impacts of different operation parameters (i.e., COD/SO42- ratio, temperature and illumination time) on the UASB performance were explored. Third, the properties of sludge derived from the UASB at different time were analyzed. Results show that after 28 days of start-up, the COD removal efficiencies in both the photoreactor and non-photoreactor could reach a range of 85-90% while such reactors could achieve > 90% of sulfate being removed. Besides, higher illumination time could facilitate the removal of pollutants in the photoreactor. To sum up, the present study can provide technical support for the clean removal of sulfate from wastewater using photoreactors.

Exosomes: efficient macrophage-related immunomodulators in chronic lung diseases.

Macrophages, the predominant immune cells in the lungs, play a pivotal role in maintaining the delicate balance of the pulmonary immune microenvironment. However, in chronic inflammatory lung diseases and lung cancer, macrophage phenotypes undergo distinct transitions, with M1-predominant macrophages promoting inflammatory damage and M2-predominant macrophages fostering cancer progression. Exosomes, as critical mediators of intercellular signaling and substance exchange, participate in pathological reshaping of macrophages during development of pulmonary inflammatory diseases and lung cancer. Specifically, in inflammatory lung diseases, exosomes promote the pro-inflammatory phenotype of macrophages, suppress the anti-inflammatory phenotype, and subsequently, exosomes released by reshaped macrophages further exacerbate inflammatory damage. In cancer, exosomes promote pro-tumor tumor-associated macrophages (TAMs); inhibit anti-tumor TAMs; and exosomes released by TAMs further enhance tumor proliferation, metastasis, and resistance to chemotherapy. Simultaneously, exosomes exhibit a dual role, holding the potential to transmit immune-modulating molecules and load therapeutic agents and offering prospects for restoring immune dysregulation in macrophages during chronic inflammatory lung diseases and lung cancer. In chronic inflammatory lung diseases, this is manifested by exosomes reshaping anti-inflammatory macrophages, inhibiting pro-inflammatory macrophages, and alleviating inflammatory damage post-reshaping. In lung cancer, exosomes reshape anti-tumor macrophages, inhibit pro-tumor macrophages, and reshaped macrophages secrete exosomes that suppress lung cancer development. Looking ahead, efficient and targeted exosome-based therapies may emerge as a promising direction for treatment of pulmonary diseases.

A rare case report: co-occurrence of two types of lung cancer with hamartoma and pulmonary tuberculosis.

With the widespread use of low-dose chest Computed Tomography (CT), lung nodules are being increasingly detected. Common pulmonary conditions such as lung adenocarcinoma, lung squamous cell carcinoma, and tuberculosis are typically diagnosable through imaging examinations. Nevertheless, when multiple types of lung cancer are combined with other benign tumors, how can an accurate diagnosis be made? In this report, we present a rare case of a patient with the simultaneous occurrence of lung adenocarcinoma, lung squamous cell carcinoma, pulmonary tuberculosis, and pulmonary hamartoma, which has not been previously reported. This patient underwent surgical intervention in the Department of Thoracic Surgery at the Second Hospital of Jilin University and has now fully recovered and been discharged. The patient's preoperative positron emission tomography-CT(PET-CT)results did not align with the postoperative pathological diagnosis. The imaging findings were atypical, and the pathological diagnosis was exceptionally rare. We share this case report to contribute to the accumulation of clinical experience.

Co-gasification of rural solid waste and biomass in rural areas: Simulation and plant-scale process.

Co-gasification technology is considered to be one of the most potential technologies for solid waste treatment, and the co-gasification treatment of rural solid waste (RSW) and biomass can effectively promote waste reduction and resource utilization. In the present study, the co-gasification of RSW and biomass in an updraft fixed bed gasifier was simulated using the Aspen Plus software, where the simulation results were validated via plant-scale experiments. In this scenario, the impacts of biomass source (i.e., rice husk, rice straw, tree bark and corn straw), co-gasification ratio (CGR) (0-40%) and air equivalence ratio (AER) (0.30-0.55) on the performance of the fixed-bed were investigated. Results showed that Aspen Plus could describe the plant-scale co-gasification process well. Besides, the tree bark-RSW system had the highest heat conversion efficiency of 6.00 MJ/kg the simulation temperature of the gasification layer increased greatly from 485 to 913 °C when the AER increased from 0.40 to 0.55. In addition, the co-gasification of RSW and tree bark could achieve the highest efficiency at the AER of 0.45 and CGR of 20% w, in which the gasification temperature reached 799 °C with the gasification efficiency of 57.17%. This study explored the use of co-gasification of RSW and biomass in rural areas by simulation and plant-scale processes, which promotes the commercial application of co-gasification technology and contributes to sustainable waste management in rural areas.

A new strategy to simultaneous removal and recovery of nitrogen from wastewater without N2O emission by heterotrophic nitrogen-assimilating bacterium.

Biological assimilation that recovery the nitrogen from wastewater in the form of biomass offers a more environmentally friendly solution for the limitations of the conventional wastewater treatments. This study reported the simultaneous removal and recovery of nitrogen from wastewater without N2O emission by a heterotrophic nitrogen-assimilating Acinetobacter sp. DN1 strain. Nitrogen balance, biomass qualitative analysis, genome and enzyme studies have been performed to illustrate the mechanism of nitrogen conversion by strain DN1. Results showed that the ammonium removal followed one direct pathway (GOGAT/GDH) and three indirect pathways (NH4+ → NH2OH → NO → NO2- → NH4+ → GOGAT/GDH; NH4+ → NH2OH → NO → NO2- → NO3- → NO2- → NH4+ → GOGAT/GDH; NH4+ → NH2OH → NO → NO3- → NO2- → NH4+ → GOGAT/GDH). Nitrogen balance and biomass qualitative analysis showed that over 70 % of the ammonium in the wastewater was converted into intracellular nitrogen-containing compounds and stored in the cells of strain DN1. Traditional denitrification pathway was not detected and the ammonium was removed through assimilation, which makes it more energy-saving for nitrogen recovery when compared with Haber-Bosch process. This study provides a new direction for simultaneous nitrogen removal and recovery without N2O emission by the heterotrophic nitrogen-assimilating bacterium.

A rare hepatic artery variant reporting and a new classification.

Variations of the hepatic artery are very common, but they greatly increase the difficulty of surgery and the risk of complications in perihepatic surgeries such as liver transplantation, liver segmentectomy, and gastroduodenal surgery. Thus, it is important to precisely define the type of hepatic artery variant before surgery. However, there are often rare variants that cannot be defined with existing classifications. For example, the type of hepatic artery variant in the current case could not be classified with conventional classifications, and no such variation has been reported to date, involving two accessory left hepatic arteries from the common hepatic and left inferior phrenic arteries, respectively. Based on the existing 3DCT technology and the CRL classification method, which is applicable to the most common hepatic artery variants, we reviewed many rare variant types and proposed a new classification method (ex-CRL classification) for hepatic artery variations that do not fit the classic scope. The ex-CRL classification can accurately classify the vast majority of rare cases in the literature, greatly compensates for the limitations of current hepatic artery classifications, improves the generalization and understanding of rare cases, and reduces surgical complications.

Design and operation of a fixed-bed pyrolysis-gasification-combustion pilot plant for rural solid waste disposal.

This paper is to explore the use of rural solid waste (RSW) for pyrolysis-gasification-combustion in pilot plant scale aiming at sustainable management of rural waste in remote areas. Based on the experimental data obtained during pilot scale operation, the temperature in the furnace needs to be kept at least at 600 °C through analyzing the pyrolysis weight loss of the main combustibles in the RSW. Besides, the effects of the air supply method and ventilation rate on the pilot plant performance were explored. Results indicate that the active air supply method positively contributes to the performance of the pilot plant. The plant processed 10 t RSW/d, producing 12.82 g/Nm3 of tar with 1.75 % of ash. This study confirms the feasibility of the pilot plant for RSW disposal and provides theoretical support for the optimization of pilot plant operation.

Photodegradation of chloramphenicol in micro-polluted water using a circulatory thin-layer inclined plate reactor.

This study describes the photodegradation of chloramphenicol (CAP) in micro-polluted water with a thin-layer inclined plate reactor. Under simulated sunlight irradiation, the effect of reaction parameters including solution pH, initial CAP concentration, and co-existed humic acid (HA) or chloride was evaluated. The photodegradation of CAP was independent of initial pH in the range of 6.0-9.0, but sharply decreased by 25.5% with the increase of initial CAP concentration from 0.4 to 1.0 mg/L. The presence of HA exhibited a significant inhibitory effect, while Cl- promoted the photoreaction. In this study, CAP was degraded through both direct and indirect photolysis, in which 1O2 was the main reactive species responsible for the indirect route. Its steady-state concentration in the micro-polluted water was determined to be 1.40 × 10-13 mol/L. Transformation intermediates were identified to propose the degradation pathway of CAP, which substantially met the density functional theory (DFT) calculation results. Moreover, four other pharmaceuticals including tetracycline, doxycycline, oxytetracycline, and minocycline were also successfully photodegraded during 5 h irradiation. Therefore, the designed circulatory thin-layer inclined plate reactor is suggested to be effectively applied to the decontamination of organic micro-polluted water.

UV-LED/chlorine degradation of propranolol in water: Degradation pathway and product toxicity.

This study reports on the propranolol (PRO) degradation performance and product toxicity of an ultraviolet light-emitting diode (UV-LED)/chlorine process. The effects of experimental parameters including solution pH, chlorine dosage, and water matrix constituents on PRO removal were evaluated. Up to 94.5% of PRO could be eliminated within 15 min at a PRO-to-chlorine molar ratio of 1:4. The overall removal efficiency of PRO was non-pH dependent in the range of 5-9, while the initial rate was accelerated under alkaline conditions. The presence of Cl-/HCO3- had little influence on the PRO degradation, whereas either humic acid or NO3- had an obvious inhibitory effect. Radical scavenger experiments showed that both HO and Cl primarily contributed to the PRO degradation, and electron paramagnetic resonance data demonstrated the generation of 1O2. The transformation of PRO during this process led to five detected products, which exhibited a higher acute toxicity than the parent compound according to the bright luminescent bacillus T3 method. It is worth mentioning that under the same ultraviolet illumination intensity, the degradation of PRO under UV-LED/chlorine gave a better performance than UV254/chlorine, but the EEO of the former is obviously higher than the latter. So further research is required on improving the electric current to photon conversion efficiency for UV-LED. Additionally, the UV-LED/chlorine system was effective in the degradation of other drugs including sulfamethoxazole, oxytetracycline hydrochloride, and gatifloxacin, suggesting the possible application of the UV-LED/chlorine process for the removal of pharmaceuticals during wastewater treatment.

Evaluation of carboxymethylpullulan-AlCl3 as a coagulant for water treatment: A case study with kaolin.

A reduction in the use of aluminum (Al)-based flocculants in the treatment of drinking water is considered essential for human health reasons. In this study, a novel composite flocculant, made of carboxymethylpullulan-AlCl3 , is evaluated in a lab-scale, jar test system for the flocculation of kaolin. The results showed that the coagulation efficiency of carboxymethylpullulan-AlCl3 was more effective in reducing turbidity than the solo use of carboxymethylpullulan or AlCl3 . The optimum treatment conditions assessed by a response surface methodology were obtained at pH 6.50, 13.03 mg/L carboxymethylpullulan, and 94.87 mg/L AlCl3 . Zeta potential measurements and photometric dispersion analysis demonstrated that AlCl3 had a more significant influence on charge neutralization than carboxymethylpullulan, whilst carboxymethylpullulan facilitated absorption and the development of particle bridges. Thus, the composite flocculant possessed both advantages that enhanced flocculation, and decreased the dosage of AlCl3 , thereby reducing the potential for secondary environment pollution. When 90 mg/L carboxymethylpullulan-AlCl3 was added to the model kaolin suspension characterized by a turbidity of 50 nephelometric turbidity units, the zeta potential and the maximum flocculating activity were determined as -2.28 mV and 98.0%, respectively. The results provide insight into the development of an environment-friendly composite flocculant prepared from water-dissolved polysaccharide and inorganic flocculants. PRACTITIONER POINTS: A novel composite flocculant CMP-AlCl3 was achieved by combining CMP and AlCl3 for water treatment. The coagulation efficiency of CMP-AlCl3 was more effective in reducing turbidity than the solo use of CMP or AlCl3 . The flocculation efficiency and mechanism were investigated by Zeta potential analysis, surface morphology, electron microscopy, and coagulation.

A low-voltage pulse electrolysis method for the degradation of anthraquinone and azo dyes in chloride medium by anodic oxidation on Ti/IrO2 -RuO2 -SnO2 electrodes.

Wastewater produced by the textile industry containing azo dyes and anthraquinone dyes is significant source of pollution to the environment and is toxic for aquatic life. To overcome the high-energy cost of traditional electrochemical oxidation, a custom-built power supply device for the degradation of anthraquinone and azo dyes by low voltage of 15.0-20.0 V pulsed discharge was investigated. Titanium coated with mixed oxide (Ti/IrO2 -RuO2 -SnO2 ) plates and pure titanium plates were used as the anode and cathode, respectively, for the generation of chlorine in the dye solution. For the anthraquinone dye Reactive Blue 19, 60.0% of the chemical oxygen demand (COD) and 22.0% of the total organic carbon (TOC) were removed using this system. A comparison of the direct current electrolysis and pulsed discharge revealed that using the pulsed discharge method reduced the energy cost by 68.6%. UV-visible, LC-MS, and GC-MS were used to identify the intermediate compounds formed during the degradation of Reactive Blue 19. The results indicate that in the process of oxidation by chlorine/hypochlorite, the chromophore group was first oxidized to -NH2 , followed by decolorization via chlorination of the aromatic rings. The results confirm that low-voltage pulse electrolysis can be used for the degradation of industrial dyes in waste effluents. PRACTITIONER POINTS: Low-voltage pulse electrolysis can be used for the degradation of industrial dyes and/or dyes in waste effluents. For anionic dye Reactive Blue 19, 60.0% of COD and 22.0% of TOC were removed using low-voltage (20.0 V) pulse electrolysis. The pulsed discharge method reduced the energy cost of this degradation process by 68.6% compared with direct current electrolysis. The intermediate compounds formed during the degradation of Reactive Blue 19 were confirmed by UV-visible spectroscopy, LC-MS, and GC-MS.

[Phosphate Removal Using Rice Husk Biochars Modified with Lanthanum Hydroxide].

La-modified RHBCs (La-RHBCs) were fabricated by immobilizing La(OH)3 nanoparticles on mesoporous rice husk biochars (RHBCs) using a co-precipitation method. Specifically, the effects of the pore structure of the RHBCs, solution pH, and coexisting substances on phosphate adsorption by the La-RHBCs were studied. The results showed that the La loading of the La-RHBCs was positively correlated with the mesoporosity of the RHBCs. La-modified RHBCs with higher mesoporosity hosts showed faster adsorption rates and lower leaching of La during phosphate adsorption. The adsorption process could be described by a pseudo second-order kinetic model, and the reaction rate was controlled by intraparticle diffusion. The Langmuir isotherm model fitted the adsorption process better, and the theoretical maximum adsorption capacities were 41.22, 43.26, and 45.62 mg·g-1, respectively. The high P/La molar ratios of more than 1.5 indicated the high utilization efficiencies of the La in the La-immobilized RHBCs. Moreover, phosphate could be effectively removed by the La-modified RHBCs over a wide pH range of 3-9. The La-modified RHBCs also exhibited good adsorption selectivity towards phosphate in the presence of coexisting anions and humic acids. Phosphate adsorption by the La-RHBCs was enhanced in the presence of Ca2+, while it was inhibited in the presence of Mg2+.

The adsorption of phosphate using a magnesia-pullulan composite: kinetics, equilibrium, and column tests.

A magnesia-pullulan (MgOP) composite has been developed to remove phosphate from a synthetic solution. In the present study, the removal of phosphate by MgOP was evaluated in both a batch and dynamic system. The batch experiments investigated the initial pH effect on the phosphate removal efficiency from pH 3 to 12 and the effect of co-existing anions. In addition, the adsorption isotherms, thermodynamics, and kinetics were also investigated. The results from the batch experiments indicate that MgOP has encouraging performance for the adsorption of phosphate, while the initial pH value (3-12) had a negligible influence on the phosphate removal efficiency. Analysis of the adsorption thermodynamics demonstrated that the phosphate removal process was endothermic and spontaneous. Investigations into the dynamics of the phosphate removal process were carried out using a fixed bed of MgOP, and the resulting breakthrough curves were used to describe the column phosphate adsorption process at various bed masses, volumetric flow rates, influent phosphate concentrations, reaction temperatures, and inlet pH values. The results suggest that the adsorption of phosphate on MgOP was improved using an increased bed mass, while the reaction temperature did not significantly affect the performance of the MgOP bed during the phosphate removal process. Furthermore, higher influent phosphate concentrations were beneficial towards increasing the column adsorption capacity for phosphate. Several mathematic models, including the Adams-Bohart, Wolboska, Yoon-Nelson, and Thomas models, were employed to fit the fixed-bed data. In addition, the effluent concentration of magnesium ions was measured and the regeneration of MgOP investigated.

Preferable phosphate removal by nano-La(III) hydroxides modified mesoporous rice husk biochars: Role of the host pore structure and point of zero charge.

Immobilizing La(OH)3 nanoparticles (NPs) to porous hosts has been widely applied to inhibiting their inherent aggregation as well as the subsequent low utilization efficiency of La. In this study, a series of rice husk biochars (RHBCs) with high mesoporous rates were prepared and the effects of host pore structure and point of zero charge (pHpzc) on phosphate adsorption by La-modified RHBCs was particularly focused. Characterization results confirmed that La(OH)3 NPs were both confined in the pore channel and external surface of RHBCs. Adsorption kinetics and isotherms showed that La-modified RHBCs with higher mesoporous rates of the host showed a faster adsorption rate and La-modified RHBCs exhibited superior La utilization efficiency than many reported La-incorporated adsorbents. Phosphate could be effectively captured over a wide pH of 3-10 due to the high pHpzc of La-modified RHBCs. Moreover, the La-modified RHBCs showed satisfactory affinity towards phosphate in the presence of coexisting anions and the phosphate adsorption by La-RHBC9 was enhanced in the presence of Ca2+, while it was inhibited in the presence of Mg2+. The mesoporous structure of RHBCs strengthened the stability of La-modified RHBCs and weakened the inhibition of coexisting humic substances on phosphate adsorption through the "shielding effect".

Fluoride removal from water using a magnesia-pullulan composite in a continuous fixed-bed column.

A magnesia-pullulan composite (MgOP) was previously shown to effectively remove fluoride from water. In the present study, a continuous fixed-bed column was used to examine the application of the composite at an industrial scale. The influencing parameters included bed mass (4.0, 6.0 and 8.0 g), influent flow rate (8, 16 and 32 mL/min), inlet fluoride concentration (5, 10 and 20 mg/L), reaction temperature (20, 30 and 40 °C), influent pH (4, 7 and 10) and other existing anions (HCO3-, SO42-, Cl- and NO3-), through which the breakthrough curves could be depicted for the experimental data analysis. The results indicated that MgOP is promising for fluoride removal with a defluoridation capacity of 16.6 mg/g at the bed mass of 6.0 g, influent flow rate of 16 mL/min and inlet fluoride concentration of 10 mg/L. The dynamics of the fluoride adsorption process were modeled using the Thomas and Yan models, in which the Yan model presented better predictions for the breakthrough curves than the Thomas model. Moreover, the concentration of magnesium in the effluent was monitored to determine Mg stability in the MgOP composite. Results indicated the effluent concentration of Mg2+ ions could be kept at a safe level. Calcination of fluoride-loaded MgOP effectively regenerated the material.

Catalytic wet air oxidation of high concentration pharmaceutical wastewater.

In this study, we investigated the pretreatment of a high concentration pharmaceutical wastewater by catalytic wet air oxidation (CWAO) process. Different experiments were conducted to investigate the effects of the catalyst type, operating temperature, initial system pH, and oxygen partial pressure on the oxidation of the wastewater. Results show that the catalysts prepared by the co-precipitation method have better catalytic activity compared to others. Chemical oxygen demand (COD) conversion increased with the increase in temperature from 160 to 220 °C and decreased with the increase in pH. Moreover, the effect of the oxygen partial pressure on the COD conversion was significant only during the first 20 min of the reaction. Furthermore, the biodegradability of the wastewater improved greatly after CWAO, the ratio of BOD5/COD increased less than 0.1-0.75 when treated at 220 °C (BOD: biochemical oxygen demand).

A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes.

BACKGROUND: To facilitate the screening of large quantities of new antimicrobial peptides (AMPs), we describe a cost-effective method for high throughput prokaryotic expression of AMPs. EDDIE, an autoproteolytic mutant of the N-terminal autoprotease, Npro, from classical swine fever virus, was selected as a fusion protein partner. The expression system was used for high-level expression of six antimicrobial peptides with different sizes: Bombinin-like peptide 7, Temporin G, hexapeptide, Combi-1, human Histatin 9, and human Histatin 6. These expressed AMPs were purified and evaluated for antimicrobial activity. RESULTS: Two or four primers were used to synthesize each AMP gene in a single step PCR. Each synthetic gene was then cloned into the pET30a/His-EDDIE-GFP vector via an in vivo recombination strategy. Each AMP was then expressed as an Npro fusion protein in Escherichia coli. The expressed fusion proteins existed as inclusion bodies in the cytoplasm and the expression levels of the six AMPs reached up to 40% of the total cell protein content. On in vitro refolding, the fusion AMPs was released from the C-terminal end of the autoprotease by self-cleavage, leaving AMPs with an authentic N terminus. The released fusion partner was easily purified by Ni-NTA chromatography. All recombinant AMPs displayed expected antimicrobial activity against E. coli, Micrococcus luteus and S. cerevisia. CONCLUSIONS: The method described in this report allows the fast synthesis of genes that are optimized for over-expression in E. coli and for the production of sufficiently large amounts of peptides for functional and structural characterization. The Npro partner system, without the need for chemical or enzymatic removal of the fusion tag, is a low-cost, efficient way of producing AMPs for characterization. The cloning method, combined with bioinformatic analyses from genome and EST sequence data, will also be useful for screening new AMPs. Plasmid pET30a/His-EDDIE-GFP also provides green/white colony selection for high-throughput recombinant AMP cloning.

Photocatalytic pretreatment of oily wastewater from the restaurant by a vacuum ultraviolet/TiO2 system.

The present study aims at investigating the performance of a vacuum ultraviolet (VUV, 185 nm) and TiO(2) oxidation system for the pretreatment of oily wastewater from restaurant. The influence of irradiation time, pH, dissolved oxygen (DO), the dosage of TiO(2) and the initial chemical oxygen demand (COD) concentration on COD removal efficiency was ascertained and optimum process conditions for stable and effective operation were determined. Under the optimum conditions of irradiation 10 min, initial COD 3981 mg/L, TiO(2) 150 mg/L, pH 7.0 and flow rate of air 40 L/h, the process of VUV and TiO(2)/VUV achieved removal efficiencies of COD, BOD(5) and oil as 50±3%, 37±2%, 86±3%, and 63±3%, 43±2%, 70±3%, respectively. The biodegradability factor f(B) of the wastewater was determined as 1.56 which indicated that the VUV/TiO(2) process improved the biodegradability of the oily wastewater significantly. Results clearly indicate that VUV/TiO(2) photolysis tends to destruct parts of COD, BOD(5), and ammonia, as well as enhances the biodegradability of the oily wastewater simultaneously. Thus, this technique could be used as a pretreatment step for conventional biological treatment of oily wastewater.